Structures of Argininosuccinate Synthetase in Enzyme-ATP-Substrates and Enzyme-AMP-Product Forms: Stereochemistry of the Catalytic Reaction. Masaru Goto, Rie Omi, Ikuko Miyahara, Mitsuaki Sugahara, and Ken Hirotsu, J. Biol. Chem. (2003) in press.
Strain and catalysis in aspartate aminotransferase. Hayashi H, Mizuguchi H, Miyahara I, Islam MM, Ikushiro H, Nakajima Y, Hirotsu K, Kagamiyama H. Biochim Biophys Acta.1647(1-2), 103-109 (2003).
Characterization of histidinol phosphate aminotransferase from Escherichia coli. Mizuguchi H, Hayashi H, Miyahara I, Hirotsu K, Kagamiyama H. Biochim. Biophys. Acta. 1647(1-2), 321-324 (2003).
Crystal structures of branched-chain amino Acid aminotransferase complexed with glutamate and glutarate: true reaction intermediate and double substrate recognition of the enzyme. Goto M, Miyahara I, Hayashi H, Kagamiyama H, Hirotsu.K. Biochemistry42(13), 3725-3733 (2003).
The active site structure of quinohemoprotein amine dehydrogenase inhibited by p-nitrophenyl-hydrazine. Atsuko Satoh, Osao Adachi, Katsuyuki Tanizawa, Ken Hirotsu, Biochimica et Biophysica Acta, 1647(1-2), 272-277 (2003).
Expression, purification and preliminary X-ray characterization of CTP synthetase from Thermus thermophilus HB8. Goto M, Omi R, Hoseki J, Nakagawa N, Miyahara I, Hirotsu K Acta Crystallogr D Biol Crystallogr.59(3), 551-553 (2003).
Expression, purification and preliminary X-ray characterization of N-acetyl-gamma-glutamyl-phosphate reductase from Thermus thermophilus HB8. Goto M, Agari Y, Omi R, Miyahara I, Hirotsu K. Acta Crystallogr. D Biol. Crystallogr.59(2), 356-358 (2003).
Conformational change in aspartate aminotransferase on substrate binding induces strain in the catalytic group and enhances catalysis. Hayashi H, Mizuguchi H, Miyahara I, Nakajima Y, Hirotsu K, Kagamiyama H. J Biol Chem.278(11):9481-9488 (2003).
原著論文／2002年
Structure of Imidazole Glycerol Phosphate Synthase from Thermus thermophilus HB8: Open-Closed Conformational Change and Ammonia Tunneling. Omi R, Mizuguchi H, Goto M, Miyahara I, Hayashi H, Kagamiyama H, Hirotsu K. J Biochem. 132(5):759-65 (2002).
Mechanism of preferential enrichment, an unusual enantiomeric resolution phenomenon caused by polymorphic transition during crystallization of mixed crystals composed of two enantiomers. Tamura R, Fujimoto D, Lepp Z, Misaki K, Miura H, Takahashi H, Ushio T, Nakai T, Hirotsu K. J. Am. Chem. Soc.124(44), 13139-53 (2002).
Crystal structure of argininosuccinate synthetase from Thermus thermophilus HB8. Structural basis for the catalytic action. Goto M, Nakajima Y, Hirotsu K. J. Biol. Chem.277(18), 15890-6 (2002).
Three-dimensional Structure of the Flavoenzyme Acyl-CoA Oxidase-II from Rat Liver, the Peroxisomal Counterpart of Mitochondrial Acyl-CoA Dehydrogenase, Yoshitaka Nakajima, Ikuko Miyahara, Ken Hirotsu, Yasuzo Nishina, Kiyoshi Shiga, Chiaki Setoyama, Haruhiko Tamaoki, and Retsu Miura, J. Biochem.131(3), 365-374 (2002).
Effects of hydrogen bonds in association with flavin and substrate in flavoenzyme D-amino acid oxidase. The catalytic and structural roles of Gly313 and Thr317. Chiaki Setoyama, Yasuzo Nishina, Haruhiko Tamaoki, Hisashi Mizutani, Ikuko Miyahara, Ken Hirotsu, Kiyoshi Shiga, and Retsu Miura, J. Biochem.131(1), 59-69 (2002).
Crystal Structure of Quinohemoprotein Amine Dehydrogenase from Pseudomonas putida, Identification of a Novel Quinone Cofactor encaged by Mutiple Thioether Cross-Brudges, Atsuko Satoh, Jong-Keun Kim, Ikuko Miyahara, Bart Devreese, Isabel Vandenberghe, Ayse Hacisalihoglu|, Toshihide Okajima, Shun'ichi Kuroda, Osao Adachi, Johannis A. Duine|, Jozef Van Beeumen, Katsuyuki Tanizawa，, and Ken Hirotsu, J. Biol. Chem. 277(4), 2830-2834 (2002).
